Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
isocitrate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
0.24 | - |
succinate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
0.4 | - |
glyoxylate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | Mycobacterium tuberculosis | - |
succinate + glyoxylate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | - |
Mycobacterium tuberculosis | succinate + glyoxylate | - |
r | |
isocitrate | isotopic analysis, solvent kinetic isotope effects in the direction of isocitrate cleavage arise from the initial deprotonation of the C2 hydroxyl group of isocitrate or the protonation of the aci-acid of the succinate product of the isocitrate aldolcleavage by a solvent-derived proton, equilibrium perturbation in D2O, NMR spectroscopy and mass spectrometry, modeling, overview. The final equilibrium isotopic composition of reactants in D2O reveals dideuterated succinate, protiated glyoxylate, and monodeuterated isocitrate, with the transient appearance and disappearance of monodeuterated succinate | Mycobacterium tuberculosis | succinate + glyoxylate | - |
r | |
succinate + glyoxylate | - |
Mycobacterium tuberculosis | isocitrate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ICL | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
succinate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
3.6 | - |
glyoxylate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis | |
5.2 | - |
isocitrate | pH 7.5, 25°C, recombinant enzyme | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | first enzyme of the glyoxylate shunt, which allows for the anaplerosis of citric acid cycle intermediates under nutrient limiting conditions | Mycobacterium tuberculosis |